2024
The Synthesis of a Specifically Isotope Labelled Fluorotryptophan and its Use in Mammalian Cell-Based Protein Expression for 19F-NMR Applications.
Chem. Comm. 2024, accepted manuscript
G. Toscano, M. Rosati, L- Barbieri, K. Maier, L. Banci, E. Luchinat, R. Konrat, R.J. Lichtenecker
Synthesis of a 13C/2H Labeled Building Block to Probe the Phosphotyrosine Interactome Using Biomolecular NMR Spectroscopy.
ChemBioChem 2024, published online, doi.org/10.1002/cbic.202400663
S. Kratzwald, Thomas C. Schwarz, K. Ledolter, M. Hlavac, M. Felkl, C.F.W. Becker, R. Konrat, R.J. Lichtenecker
Characterization of conformational states of the homodimeric enzyme fluoroacetate dehalogenase by 19F-13C two-dimensional NMR.
RSC Chem. Biol. 2024, published online, doi: 10.1039/d4cb00176a
M. Suleiman, G.A. Frere, R. Törner, L. Tabunar, G.V. Bohle, K. Taverner, N. Tsuchimura, D. Pichugin, R.J. Lichtenecker, O. Vozny, P. Gunning, H. Arthanari, R.S. Prosser
Towards cost-effective-chain isotope labelling of proteins expressed in human cells.
J. Biomol. NMR 2024, published online 22.8.2024, doi.org/10.1007/s10858-024-00447-6
M. Rosati, L. Barbieri, M. Hlavac, S. Kratzwald, R.J. Lichtenecker, R. Konrat, E. Luchinat and L. Banci
https://link.springer.com/article/10.1007/s10858-024-00447-6#Abs1
Probing Protein-Ligand Methyl-Pi Interaction Geometries through Chemical Shift Measurements of Selectively Labeled Methyl Groups.
J. Med. Chem. 2024, 67, 15, 13187.
A. Beier, G. Platzer, T. Höfurthner, A.L. Ptaszek, R.J. Lichtenecker, L. Geist, J.E. Fuchs, D.B. McConnell, M. Mayer and R. Konrat
https://pubs.acs.org/doi/10.1021/acs.jmedchem.4c01128
Exploring the pH dependence of an improved PETase.
Biophys. J. 2024, online, doi.org/10.1016/j.bpj.2024.04.026
C. Charlier, S. Gavalda, J. Grga, L. Perrot, V. Gabrielli, F. Löhr, J. Schörghuber, R. Lichtenecker, G. Arnal, A. Marty, V. Tournier, G. Lippens*
https://www.cell.com/biophysj/abstract/S0006-3495(24)00288-1
Decorating phenylalanine side-chains with triple labeled 13C/19F/2H isotope patterns.
J. Biomol. NMR 2024, online, doi.org/10.1007/s10858-024-00440-z
G. Toscano, J. Holzinger, B. Nagl, G. Kontaxis, H. Kählig, R. Konrat, R.J. Lichtenecker*
13Cβ-Valine and 13Cγ-Leucine Methine Labeling To Probe Protein Ligand Interaction.
ChemBioChem 2024, online, doi.org/10.1002/cbic.202300762
G. Toscano, T. Höfurthner, B. Nagl, A. Beier, M. Mayer, L. Geist, D.B. McConnell, H. Weinstabl, R. Konrat*, R.J. Lichtenecker*
2023
Synthesis of a 13C-methylene-labeled isoleucine precursor as a useful tool for studying protein side-chain interactions and dynamics
J. Biomol. NMR 2023, online, doi.org/10.1007/s10858-023-00427-2
T. Höfurthner, G. Toscano, G. Kontaxis, A, Beier, M. Mayer, L. Geist, DB. McConnell, H. Weinstabl, R. Lichtenecker*, R. Konrat*
https://link.springer.com/article/10.1007/s10858-023-00427-2
E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation
J. Biomol. NMR 2023, 77, 131-147.
R. Levin, F. Löhr, B. Karakoc, R. Lichtenecker, V. Dötsch, F. Bernhard*
https://link.springer.com/article/10.1007/s10858-023-00417-4
The rigid core and flexible surface of amyloid fibrils probed by Magic-Angle Spinning NMR of aromatic residues
Angew. Chem. Int. Ed. 2023, 62, 19, e202219314
L.M. Becker, M. Berbon, A. Vallet, A. Grelard, E. Morvan, B. Bardiaux, R. Lichtenecker, M. Ernst, A. Loquet, P. Schanda*
Aromatic ring flips in differently packed ubiquitin protein crystals from MAS NMR and MD
J. Struct. Biol.:X 2023, 7, 100079.
D.F. Gauto, O.O. Lebedenko, L.M. Becker, I. Ayala, R. Lichtenecker, N.R. Skrynnikov, P. Schanda*
2021
The hydrogen1 R1rho relaxation dispersion experiments in aromatic side chains
J. Biomol. NMR 2021, 75, 383-392.
M. Dreydoppel, R.J. Lichtenecker, M. Akke, U. Weininger*
1H R1ρ relaxation dispersion experiments in aromatic side chains | SpringerLink
2020
PI by NMR: Probing CH-Pi Interactions in Protein-Ligand Complexes by NMR Spectroscopy.
Angew. Chem. Int. Ed. 2020, 59, 14861-14868.
G. Platzer, M. Mayer, A. Beier, S. Brueschweiler, J.E. Fuchs, H. Engelhardt, L. Geist, G. Bader, J. Schörghuber, R. Lichtenecker, B. Wolkerstorfer, D. Kessler, D.B. McConnell*, R. Konrat*
https://doi.org/10.1002/anie.202003732
2019
Site-selective 1H/2H labeling enables artifact-free 1H CPMG relaxation dispersion experiments in aromatic side chains.
J. Biomol. NMR 2019, 73, 633-639.
H.N. Raum, J. Schörghuber, M. Dreydoppel, R.J. Lichtenecker, U. Weininger*
https://doi.org/10.1007/s10858-019-00275-z
Aromatic ring dynamics, thermal activation and transient conformations of a 468 kDa enzyme by specific labeling and fast-MAS NMR.
J. Am. Chem. Soc. 2019, 141, 28, 11183-11195.
D.F. Gauto, P. Macek, A. Barducci, H. Fraga, A. Hessel, T. Terauchi, D. Gajan, Y. Miyanoiri, J. Boisbouvier, R.J. Lichtenecker, M. Kainosho, P. Schanda*
https://doi.org/10.1021/jacs.9b04219
Futile encounter engineering of the DSR-M dextransucrase modifies the resulting polymer length.
Biochemistry 2019, 58, 25, 2853-2859.
Claverie M, Cioci G, Guionnet M, Schörghuber J, Lichtenecker R, Moulis C, Remaud-Simeon M*, Lippens G*
https://pubs.acs.org/doi/full/10.1021/acs.biochem.9b00373
2018
Late metabolic precursors for selective aromatic residue labeling.
J Biomol NMR 2018, 71, 3, 129-140.
Schörghuber J, Geist L, Platzer G, Feichtinger M, Bisaccia M, Scheibelberger L, Weber F, Lichtenecker RJ*
https://link.springer.com/article/10.1007/s10858-018-0188-z
2017
Anthranilic acid, the new player in the ensemble of aromatic residue labeling precursor compounds.
J Biomol NMR 2017, 69, 1, 13-22.
Schörghuber J, Geist L, Bisaccia M, Weber F, Konrat R, Lichtenecker RJ*
https://link.springer.com/article/10.1007/s10858-017-0129-2
Highly selective stable isotope labeling of histidine residues using a novel precursor in E. coli-based overexpression systems.
ChemBioChem 2017, 18, 1487–1491.
Schörghuber J, Geist L, Platzer G, Konrat R, Lichtenecker RJ*
onlinelibrary.wiley.com/doi/10.1002/cbic.201700192/full
Bioactivity of topologically confined gramicidin A dimers.
Bioorg. Med. Chem. 2017, 25, 261-268.
Jadhav KB, Stein C, Makarewicz O, Pradel G, Lichtenecker RJ, Sack H, Heinemann SH, Arndt H-D*
www.sciencedirect.com/science/article/pii/S0968089616310781
2015
Synthesis of Metabolic Amino acid Precursors: Tools for Selective Isotope Labeling in Cell-Based Protein Overexpression.
Synlett 2015, 26, 2611-2616.
Lichtenecker RJ*, Schörghuber J, Bisaccia M
www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0034-1378878
Novel approaches in selective tryptophan isotope labeling using Escherichia coli overexpression media.
ChemBioChem 2015, 16, 5, 746-751.
Schörghuber J, Sara T, Bisaccia M, Schmid W, Konrat R, Lichtenecker RJ*
onlinelibrary.wiley.com/doi/10.1002/cbic.201402677/full
Dynamic combinatorial enrichment of polyconformational D-/L peptide dimers.
Chem. Eur. J. 2015, 21, 15, 5898-5908.
Jadhav KBǂ, Lichtenecker RJǂ, Bullach A, Bhubaneswar M, Arndt H-D* ǂ equal contribution
onlinelibrary.wiley.com/doi/10.1002/chem.201405413/full
2014
Synthesis of aromatic 13C/2H-α-ketoacid precursors to be used in selective phenylalanine and tyrosine protein labeling.
Org. Biomol. Chem. 2014, 12, 7551-7560.
Lichtenecker RJ*
pubs.rsc.org/en/content/articlehtml/2014/ob/c4ob01129e
2013
α-Ketoacids as precursors for phenylalanine and tyrosine labelling in cell-based protein overexpression.
J. Biomol. NMR 2013 57(4): 327-331.
Lichtenecker RJ*, Weinhaeupl K, Schmid W, Konrat R
link.springer.com/article/10.1007/s10858-013-9796-9
Independent valine and leucine isotope labeling in Escherichia coli protein overexpression systems.
J. Biomol. NMR 2013 57(3): 205-209.
Lichtenecker RJ*, Weinhaeupl K, Reuther L, Schoerghuber J, Schmid W, Konrat R
link.springer.com/article/10.1007/s10858-013-9786-y/fulltext.html
Iterative Antimicrobial Candidate Selection from Informed D-/L-Peptide Dimer Libraries.
ChemBioChem. 2013 14(18): 2492-2499.
Lichtenecker RJ, Ellinger B, Han H-M, Jadhav KB, Baumann S, Makarewicz O, Grabenbauer M, Arndt H-D*
onlinelibrary.wiley.com/doi/10.1002/cbic.201300243/full
Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds.
ChemBioChem. 2013 14:818-21.
Lichtenecker RJ*, Coudevylle N, Konrat R, Schmid W
onlinelibrary.wiley.com/doi/10.1002/cbic.201200737/full
<2011
Biomimetic synthesis of azole- and aryl-peptide alkaloids.
Biomimetic Organic Synthesis (Edited by E. Poupon, B. Nay), Wiley 2011 1: 317-355.
Arndt H-D*, Lichtenecker R, Loos P, Milroy L-G
Application of various ionic liquids as cosolvents for chloroperoxidase-catalysed biotransformations.
Monatsh. Chem. 2009 140(5): 509-512.
Lichtenecker RJ, Schmid W*
Direct methods and residue type specific isotope labeling in NMR structure determination and model-driven sequential assignment.
J. Biomol. NMR 2008 42(2): 111-127.
Schedlbauer A, Auer R, Ledolter K, Tollinger M, Kloiber K, Lichtenecker R, Ruedisser S, Hommel U, Schmid W, Konrat R, Kontaxis G*
An Isolated Helix Persists in a Sparsely Populated Form of KIX under Native Conditions.
Biochemistry 2006 45(29): 8885-8893.
Tollinger M*, Kloiber K, Agoston B, Dorigoni C, Lichtenecker R, Schmid W, Konrat R
Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing.
J. Am. Chem. Soc. 2004 126(17): 5348-5349.
Lichtenecker R, Ludwiczek ML, Schmid W, Konrat R*
Tuning of Redox Potentials for the Design of Ruthenium Anticancer Drugs – an Electrochemical Study of [trans-RuCl4L(DMSO)]- and [trans-RuCl4L2]- Complexes, where L = Imidazole, 1,2,4-Triazole, Indazole.
Inorganic Chemistry 2004 43(22): 7083-7093.
Reisner E, Arion VB*, Guedes da Silva MFC, Lichtenecker R, Eichinger A, Keppler BK*, Vadim Y, Pombeiro AJL*
Two novel trans-tetrachloro(dimethyl sulfoxide)azoleruthenate(III) complexes, their antiproliferative activity and the coordination mode of triazole in H2trz[RuCl4(dmso)(Htrz)]
Inorg. Biochem. 2003, 96, 1, 243.
Unfried P*, Reisner E, Fremuth M, Jakupec MA, Lichtenecker R, Arion VB, Keppler BK J.